TY - JOUR
T1 - Evaluation of the Impact of Securinega virosa Leaf Extract on Methicillin-Resistant Staphylococcus aureus
AU - Wei Chen
AU - Li Zhang
AU - Qiang Sun
JF - EAMD 3
Y1 - 0
VL - 0
IS - 0
SP - 4
N2 - This research focused on comparing the effects of Securinega virosa leaf extract and its partitioned fractions against methicillin-resistant Staphylococcus aureus (MRSA). The extraction process utilized 80% methanol, and the resulting crude extract underwent phytochemical screening using established protocols. Antimicrobial potency of the crude methanol extract and its aqueous and chloroform fractions was assessed using the agar well diffusion method. Additionally, their minimum inhibitory concentrations were determined. The phytochemical analysis revealed that the leaf extract of S. virosa contained various bioactive compounds, including alkaloids, flavonoids, saponins, steroids, tannins, terpenes, cardiac glycosides, anthraquinones, and reducing sugars. When tested for antibacterial activity, the crude extract at concentrations of 100, 50, 25, 12.5, and 6.25 mg/ml produced inhibition zones averaging 14.67 ± 0.82, 10.33 ± 0.82, 8.33 ± 0.41, 6.67 ± 0.41, and 4.33 ± 0.82 mm, respectively. For the partitioned fractions, the aqueous extract yielded inhibition zones of 10.50 ± 0.35, 6.67 ± 1.47, 4.67 ± 0.82, 1.67 ± 2.04, and 1.33 ± 1.63 mm across the same concentrations. The chloroform fraction resulted in inhibition zones of 10.33 ± 1.08, 6.67 ± 0.41, 4.33 ± 1.08, and 2.66 ± 1.78 mm, respectively.Regarding the minimum inhibitory concentration, the crude extract displayed effectiveness starting at 25 mg/ml, while both the aqueous and chloroform fractions showed inhibitory activity at 50 mg/ml. Overall, all forms of S. virosa extracts tested (crude methanol, aqueous, and chloroform) demonstrated activity against MRSA, with the crude extract exhibiting the most pronounced antibacterial effect.
UR - https://pubsys.eshragh.co/j112310613
ER -