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Measuring trace element concentrations in human hair serves as an important analytical method for screening potential deficiencies, excesses, or biochemical imbalances of these microelements in the body. In this research, using an inductively coupled plasma–mass spectrometry (ICP-MS) analyzer, the authors aimed to identify toxic trace elements (Al, Pb, Hg) and quantify key mineral elements (Ca, Mg, Cu, Zn) in healthy individuals with good nutritional status. The study included a sample of 75 adult women aged 30–35 years from various regions across the country. Hair samples weighing 100 mg and measuring 3 cm from the scalp base were collected for trace element analysis and evaluation. Results showed that 12 participants (16%) exhibited elevated intracellular Mg levels (1.2 mmol/L), increased Ca levels (0.72 mmol/L), yet decreased mean Ca/Mg ratios (0.58). Conversely, 6 participants (8%) demonstrated low mean values of Mg (0.004 mmol/L) and Ca (0.04 mmol/L) but had a high Ca/Mg ratio. At the time of analysis, none of the subjects displayed signs of acute or severe heavy metal intoxication. The hair trace element content reflected the environmental and lifestyle factors of the individuals from different regions of the country.